Electrophoresis Migration Calculator
29. Electrophoresis Migration
Estimate migration distance based on DNA size.
Scientific Documentation
In agarose gel electrophoresis, the migration rate of DNA fragments is inversely proportional to the log of their molecular weight. Smaller fragments move faster through the porous matrix, while larger ones are impeded.
The Principle: Migration distance ($d$) is a function of voltage ($V$), time ($t$), and the molecular weight ($MW$). While highly dependent on the agarose percentage, the semi-logarithmic relationship follows $log(MW) \propto 1/d$. This tool provides a linear approximation for standard 1% agarose gels.
Laboratory Significance: Predicting migration helps in designing gels that ensure optimal separation of target bands from contaminants. It is a critical planning step for preparative electrophoresis, ensuring that bands of interest are sufficiently separated for clean excision and downstream purification.
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