Dilution Calculator (C1V1 = C2V2)
7. Dilution Calculator (C1V1 = C2V2)
Find the required volume of stock solution for a specific concentration.
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Scientific Documentation
The C1V1 = C2V2 equation is the foundational mathematical principle of laboratory dilutions. It allows researchers to convert a high-concentration "stock" solution into a dilute "working" solution.
Variables: C1 is your starting concentration, V1 is the volume of stock you need to pipette out, C2 is your desired final concentration, and V2 is your final target volume. The volume of solvent (e.g., water or buffer) to add is calculated as: Solvent Volume = V2 - V1.
Application: In molecular biology, this is essential for tasks like creating buffers (e.g., diluting 10X PBS to 1X), preparing antibiotic working concentrations (like Ampicillin or Kanamycin for cell culture), or setting up reaction master mixes. Precision here is non-negotiable; even minor errors in V1 calculation can lead to osmotic imbalances, failed PCR reactions, or improper protein stabilization. This tool eliminates manual calculation errors, ensuring rigorous experimental consistency across laboratory protocols.
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